Journal: Cell Death & Disease
Article Title: A novel TGFbeta/TGILR axis mediates crosstalk between cancer-associated fibroblasts and tumor cells to drive gastric cancer progression
doi: 10.1038/s41419-024-06744-0
Figure Lengend Snippet: a , b The overexpression efficiency of TARBP2 was examined by western blotting assay. c , d TARBP2 overexpression significantly inhibits the proliferation and invasion of GC cell lines. e The microRNA profiling array was performed to identify the miRNAs regulated by TARBP2 overexpression in the AGS cell line. f The expression correlation between TARBP2 and per microRNA in TCGA stomach cancer cohort were shown in the volcano plot. TARBP2 was highly co-expressed with miR-1306 and miR-33a in GC. g The overall survival analysis of miR-1306 and miR-33a in the TCGA_STAD cohort. h , i TARBP2 overexpression significantly upregulated the expression levels of miR-1306 and miR-33a in GC cell lines. j The rescue assay confirmed that inhibition of miR-1306 and miR-33a can partially restore the inhibitory effect of TARBP2 overexpression on GC cell proliferation. The inhibitor-poor means the mixture of miR-1306 inhibitor and miR-33a inhibitor.
Article Snippet: Thus, a high-throughput Agilent microRNA profile microarray was performed to identify the differentially expressed microRNAs after TARBP2 overexpression.
Techniques: Over Expression, Western Blot, Expressing, Rescue Assay, Inhibition